Wearing protective masks is still impacting our skin health. Reducing inflammatory acne induced by Cutibacterium acnes remains a primary target to fight maskne. To support its customers in defining the efficacy of innovative ingredients, StratiCELL is continuously increasing its panel of anti-inflammatory in vitro assays. In its MicroBIOS Platform, StratiCELL colonizes 3D reconstructed human epidermis with living phylotype IA1 acne strain. To gain insight on this model, StratiCELL sought to define the total epidermal gene expression profile of the epidermis under the influence of C. acnes. Using a whole genome transcriptomic microarrays analysis combined to its proprietary Skin Knowledge Database, StratiCELL identified the influence of C. acnes on the expression of various key biomarkers of inflammation, innate immunity and barrier integrity. Expression of those biomarkers at the protein level was in concordance. Based on those conclusions, the Belgian CRO allows the objectivation of anti-acne ingredients by measuring their ability to modulate key biomarkers of inflammation, innate immunity and barrier integrity.