Balneology reworked

Heilmoor extract effects on microbiome balancing, skin barrier enhancement, anti-inflammatory and wound healing properties were investigated using HaCaT cells and RHE. Molecular analyses via Western blot and MSD multiplexing examined Heilmoor extract-treated cells. Its impact on aging and oxidative stress was assessed in NHDF and RAW cells. Heilmoor extract, dissolved in PBS or DMSO at 100mg/mL and 200mg/mL respectively, showed promising therapeutic potential.

Heilmoor extract possesses antimicrobial activity against a range of pathogens that are implicated in skin disease. It promotes the growth of important bacteria such as Staphylococcus epidermidis, inhibits Staphylococcus aureus growth. Additionally, it maintains vitality and diversity of the skin microbiome.

Heilmoor extract has anti-aging properties. It modulates cellular senescence and promotes wound healing. It reduces the burden of senescent cells, improves the ability to heal wounds and modulates keratinocyte differentiation markers, including the expression of K10 and K16. Iulates expression of E-cadherin B1 and B2 and inhibits sphingomyelinase activity, which also contributes to skin integrity and barrier function.

Heilmoor extract counteracts oxidative stress by reducing the production of reactive oxygen species (ROS). Agerelated functional losses and diseases are associated with accumulation of reactive oxygen (ROS) and nitrogen species, such as nitrogen monoxide (NO) Pre-clinical studies have shown a significant reduction in ROS production, 8-isoprostane levels and RAGE expression.

As evidenced by its ability to modulate the release of pro-inflammatory cytokines, Heilmoor extract has potent anti-inflammatory activity. A continuous expression of cytokines following noxious stress causes chronic inflammation and skin damage which in turn leads to skin disease and (premature) aging. In vitro studies have shown a significant reduction in the release of IL-6, IL-8, TNF-α, IL-1β, PGE2, MCP-1 and MMP9.

Rinse-off treatments are a cornerstone of the skincare routine. They provide a convenient and effective way to cleanse and revitalise the skin. Rinse-off formulations play a crucial role in maintaining skin health and vitality, whether it’s pollutants, impurities, excess oil, or acne-causing bacteria. The efficacy of Heilmoor extract in rinse-off formulations should be fully investigated to unlock its detoxifying, purifying, moisturising, soothing and acne-fighting potential.

This study investigates the immediate benefits of Heilmoor extract through a single application mask, focusing on hydration enhancement and reduction of crow’s feet wrinkles. The efficacy of the product was assessed 15 minutes post-application, evaluating both the hydration levels and surface appearance of crow’s feet wrinkles. Results demonstrate significant improvements in skin appearance, with a reduction in fine lines of up to –18.2% compared to baseline (T0), and –9.4% compared to a placebo (p<0.001) (Figure 1). Moreover, an immediate hydration effect was observed 15 minutes post-application, with an increase of +19.1% compared to baseline, and +16% compared to the placebo (p<0.001) (Figure 2a, 2b, 2c). These findings underscore the rapid and tangible benefits of Heilmoor extract in enhancing skin hydration and reducing the signs of aging, offering promising implications for immediate skincare solutions.

Sensitive skin needs gentle but effective measures to calm irritation and restore balance. The efficacy of Heilmoor extract in rinse-off formulations was demonstrated in a clinical study on sensitive skin (p<0.001). A single application of 1.5% Heilmoor extract produced a remarkable soothing effect. It was 16% better than placebo after just one minute. This effect was sustained and improved by 42% after 15 minutes (Figure 3a, 3b, 3c). This demonstrates the rapid and sustained efficacy in soothing sensitive skin.

A key role in the development of acne is played by the activation of innate immunity through the expression of cytokines by keratinocytes, leading to hyperkeratinisation of the pilosebaceous unit.

Environmental pollutants are a major threat to skin health. Effective detoxification strategies are needed. Heilmoor extract has shown remarkable efficacy in rinse-off formulations in a clinical study on detoxification and pollution protection. A single application of 1.5% Heilmoor extract resulted in a remarkable improvement in detoxification, 16.3% higher than placebo (p<0.006). The deposition of carbon microparticles on the skin was eliminated. This underlines Heilmoor extract‘s ability to cleanse, purify and protect the skin from environmental aggressors. No clinical signs of discomfort or aggravation were reported by study participants. Acne is a widespread dermatological problem. Targeted interventions are required to address its multiple causes. The effectiveness of Heilmoor extract in combating acne has been thoroughly investigated in a comprehensive 21-day study. Significant improvements in key parameters were observed with a cleansing formula containing 1.5% Heilmoor extract (Figure 4a, 4b, 4c). Compared to the placebo, sebum production was reduced by 22% (p<0.05), while open comedones and papules were reduced by 19% and 24% respectively (p<0.001) vs T0. Heilmoor extract is a promising solution for acne-prone skin due to this strong anti-acne effect.

The rinse-off studies that have investigated the effectiveness of Heilmoor extract underline its many benefits for skin care. Alpin Heilmoor Extract (AHE) offers a comprehensive solution to a variety of dermatological concerns, from detoxification and impurity protection to hydration, soothing and acne reduction. As research continues to explore its therapeutic mechanisms and clinical applications, Heilmoor extract is ready to redefine skincare and usher in a new era of holistic wellness and glowing beauty.

graphics and pictures: Premium Organi

References:

1 Gomes C, Carretero M, Pozo M, Maraver F, Cantista P, Armijo F, et al. Peloids and pelotherapy: Historical evolution, classification and glossary. Appl Clay Sci. 2013 May 1;75–76:28–38.

2 Kumar V, Bouameur J-E, Bär J, Rice RH, Hornig-Do H-T, Roop DR, et al. A keratin scaffold regulates epidermal barrier formation, mitochondrial lipid composition, and activity. J Cell Biol [Internet]. 2015 Dec 7;211(5):1057–75. Available from: https://pubmed.ncbi.nlm.nih. gov/26644517.

3 Borg M, Brincat S, Camilleri G, Schembri-Wismayer P, Brincat M, Calleja-Agius J. The role of cytokines in skin aging. Climacteric. 2013 Oct;16(5):514–21.

4 Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stress, aging, and diseases. Clin Interv Aging [Internet]. 2018 Apr 26;13:757–72. Available from: https://pubmed.ncbi.nlm.nih. gov/29731617

5 Montuschi P, Barnes PJ, Roberts LJ 2nd. Isoprostanes: markers and mediators of oxidative stress. FASEB J Off Publ Fed Am Soc Exp Biol. 2004 Dec;18(15):1791–800.

6 Shim JH. Prostaglandin E2 Induces Skin Aging via E-Prostanoid 1 in Normal Human Dermal Fibroblasts. Int J Mol Sci [Internet]. 2019 Nov 720(22):5555. Available from: https://pubmed.ncbi.nlm.nih. gov/31703303.

7 Peiris H, Dubach D, Jessup CF, Unterweger P, Raghupathi R, Muyderman H, et al. RCAN1 regulates mitochondrial function and increases susceptibility to oxidative stress in mammalian cells. Oxid Med Cell Longev [Internet]. 2014/06/09. 2014;2014:520316. Available from: https://pubmed.ncbi.nlm.nih.gov/25009690.

8 Zhang S, Duan E. Fighting against Skin Aging: The Way from Bench to Bedside. Cell Transplant [Internet]. 2018/04/25. 2018 May;27(5):729– 38. Available from: https://pubmed.ncbi.nlm.nih.gov/29692196.

9 Lohwasser C, Neureiter D, Weigle B, Kirchner T, Schuppan D. The receptor for advanced glycation end products is highly expressed in the skin and upregulated by advanced glycation end products and tumor necrosis factor-alpha. J Invest Dermatol. 2006 Feb;126(2):291–9.

10 Shen C-Y, Lu C-H, Wu C-H, Li K-J, Kuo Y-M, Hsieh S-C, Yu C-L. The Development of Maillard Reaction, and Advanced Glycation End Product (AGE)-Receptor for AGE (RAGE) Signaling Inhibitors as Novel Therapeutic Strategies for Patients with AGE- Related Diseases [Internet]. 2020 Nov 27;25(23): 5591. Available from: https://www.ncbi. nlm.nih.gov/pmc/articles/PMC7729569.

11 Li L, Hartley R, Reiss B, Sun Y, Pu J, Wu D, et al. E-cadherin plays an essential role in collective directional migration of large epithelial sheets. Cell Mol Life Sci [Internet]. 2012;69(16):2779–89. Available from: https://doi.org/10.1007/s00018-012-0951-3.

12 Shin J-W, Kwon S-H, Choi J-Y, Na J-I, Huh C-H, Choi H-R, et al. Molecular Mechanisms of Dermal Aging and Antiaging Approaches. Int J Mol Sci. 2019 Apr;20(9).

13 Dréno B. What is new in the pathophysiology of acne, an overview. J Eur Acad Dermatol Venereol. 2017 Sep;31 Suppl 5:8–12